Screening of Bacteriocin-Producing Lactic Acid Bacteria Isolated from West Algerian Goat’s Milk

Fifty nine lactic acid bacteria isolated from Algerian goat’s milk and previously selected according to their technological properties. They were screened for antimicrobial activity. From 3258 bacterial couples brought into the study, we observed 747 cases of inhibitions (22.93%). The results obtained showed that the lactobacilli have high spectrum of action. Thus, for the various species: Lc. lactis subsp. lactis (40.1%); Lc. lactis subsp. lactis biovar. diacetylactis (29.1%); Leuconostoc (25.8%); Streptococcus (56.9%); Pediococcus (36.5%) and Lactobacillus (13.5%). For the whole of the interactions brought into studies, the lactobacilli inhibited 392 strains of 1331 couples (29.5%); the other species have a more reduced spectrum of action: Lactococcus (18.76%); Leuconostoc (25.7%); Streptococcus (17.5%); and Pediococcus (5.34%). Six strains showed inhibitory activity in solid medium (well diffusion assay) when tested against the effects of organic acid and hydrogen peroxide were eliminated. These strains did not show inhibitory activity after treatment with proteinase K, trypsin, or -chymotrypsin. Lactococcus lactis subsp. lactis LCL01 produced a heat stable substance with a proteinaceous nature and with bactericidal action, suggesting a bacteriocin-like. Lactobacillus plantarum LBP01 act positively on Escherichia coli by producing a bacteriocin.